Figure 4. The novel C-terminal region between amino acids 2042 and 2061 regulates size of the fast and total releasable pool and synaptic vesicle release kinetics.
(A–B) Average release rate trace after 3 ms (A) or 30 ms stimulation (B) from calyces expressing either Cre + full transcript rescue (grey), Δ2042–2368 (green) or Δ2016–2368 (blue); n = 10 for each group; (C) Averaged cumulative release after 3 ms and 30 ms stimulation. (D) Normalized cumulative release of the total releasable pool triggered by 30 ms stimulation. Inset presents a magnified view of the area encircled by the dashed box. (E–G) Quantification of SV numbers released by 3 ms (E) and 30 ms (F) as well as the ratio of SVs released by 3 ms and 30 ms stimulation (G). All data are depicted as mean ± SEM.