Table 1.
Top proteins pulled down by biotinylated ERX-11 in MCF-7 cells, as identified by IP-MS. The column marked E2 represents spectral counts for the protein bound to biotinylated control eluted from avidin column, under conditions of E2 stimulation. The column marked E2 +ERX-11 represents spectral counts for proteins bound to biotinylated ERX-11 eluted from an avidin column with E2 stimulation. The column marked E2 + ERX-11/E2 represents the ratio of binders.
| Protein | Description | Length (AA) | Mw (Da) | PSMs | Peptide seqs | % Coverage | E2 | E2 + ERX-11 |
E2 +
ERX-11/E2 |
|---|---|---|---|---|---|---|---|---|---|
| P38117 | ETFB_HUMAN Electron transfer flavoprotein subunit beta OS = Homo sapiens GN = ETFB PE = 1 SV = 3 | 255 | 37501.10 | 12 | 7 | 34.10 | 1 | 7.00 | 5.00 |
| Q96PZ0 | PUS7_HUMAN Pseudouridylate synthase seven homolog OS = Homo sapiens GN = PUS7 PE = 1 SV = 2 | 661 | 75186.30 | 20 | 14 | 26.30 | 1.00 | 7.98 | 10.99 |
| O95336 | 6 PGL_HUMAN 6-phosphogluconolactonase OS = Homo sapiens GN = PGLS PE = 1 SV = 2 | 258 | 27601.60 | 13 | 8 | 41.50 | 0.99 | 6.00 | 6.04 |
| Q8TD06 | AGR3_HUMAN Anterior gradient protein three homolog OS = Homo sapiens GN = AGR3 PE = 1 SV = 1 | 166 | 19194.90 | 14 | 9 | 47.00 | 0.99 | 5.96 | 7.03 |
| P18754 | RCC1_HUMAN Regulator of chromosome condensation OS = Homo sapiens GN = RCC1 PE = 1 SV = 1 | 421 | 48241.20 | 27 | 13 | 55.30 | 1.99 | 11.92 | 7.00 |
| O60506 | HNRPQ_HUMAN Heterogeneous nuclear ribonucleoprotein Q OS = Homo sapiens GN = SYNCRIP PE = 1 SV = 2 | 623 | 69739.70 | 20 | 20 | 48.50 | 1.97 | 10.82 | 3.50 |
| P03372 | ESR1_HUMAN Estrogen receptor OS = Homo sapiens GN = ESR1 PE = 1 SV = 2 | 595 | 66335.20 | 36 | 15 | 30.80 | 1.83 | 9.88 | 5.93 |
| E9PCR7 | E9PCR7_HUMAN 2-oxoglutarate dehydrogenase, mitochondrial OS = Homo sapiens GN = OGDH PE = 2 SV = 1 | 1038 | 115728.00 | 39 | 25 | 32.80 | 3.98 | 18.88 | 4.00 |
| O43488 | ARK72_HUMAN Aflatoxin B1 aldehyde reductase member 2 OS = Homo sapiens GN = AKR7A2 PE = 1 SV = 3 | 359 | 39653.80 | 22 | 11 | 39.80 | 1.99 | 8.96 | 5.51 |
| O95994 | AGR2_HUMAN Anterior gradient protein two homolog OS = Homo sapiens GN = AGR2 PE = 1 SV = 1 | 175 | 22277.70 | 29 | 12 | 65.70 | 2.97 | 11.92 | 4.68 |
| P19338 | NUCL_HUMAN Nucleolin OS = Homo sapiens GN = NCL PE = 1 SV = 3 | 710 | 76766.50 | 99 | 35 | 48.00 | 14.00 | 50.98 | 2.43 |
| O43148 | MCES_HUMAN mRNA cap guanine-N7 methyltransferase OS = Homo sapiens GN = RNMT PE = 1 SV = 1 | 476 | 57831.90 | 16 | 9 | 29.40 | 1.99 | 6.97 | 3.50 |
| Q562R1 | ACTBL_HUMAN Beta-actin-like protein 2 OS = Homo sapiens GN = ACTBL2 PE = 1 SV = 2 | 376 | 42084.00 | 14 | 14 | 39.10 | 2.00 | 7.00 | 3.00 |
| Q9Y5A9 | YTHD2_HUMAN YTH domain family protein 2 OS = Homo sapiens GN = YTHDF2 PE = 1 SV = 2 | 579 | 62457.80 | 15 | 12 | 23.10 | 2.00 | 6.99 | 3.00 |
| P16152 | CBR1_HUMAN Carbonyl reductase [NADPH] 1 OS = Homo sapiens GN = CBR1 PE = 1 SV = 3 | 277 | 30427.90 | 20 | 11 | 56.70 | 2.99 | 9.98 | 2.33 |
| Q9UBS4 | DJB11_HUMAN DnaJ homolog subfamily B member 11 OS = Homo sapiens GN = DNAJB11 PE = 1 SV = 1 | 358 | 40578.70 | 21 | 12 | 35.20 | 3.00 | 10.00 | 2.67 |