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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: J Immunol. 2016 Feb 5;196(6):2583–2590. doi: 10.4049/jimmunol.1502132

Figure 4. RasGRP1-dependent signaling contributes to constitutive surface antigen expression in B1a cells.

Figure 4

(A–C) RasGRP1-dependent signaling contributes to constitutive B1 cell activation. Peritoneal B1a cells (CD19+CD5+B220low-neg) from WT control mice (red lines) and RasGRP1-deficient mice (blue lines) were stained with antibodies against CD86 (A), CD80 (B), or CD44 (C) followed by flow cytometric analysis. Shaded areas represent isotype controls. (D) RasGRP1-dependent signaling contributes to Mac-1 expression. Peritoneal B1a cells (CD19+CD5+B220low-neg) from WT control mice (red lines) and RasGRP1-deficient mice (blue lines) were stained with antibodies against Mac-1 followed by flow cytometric analysis. Shaded areas represent isotype control. Results represent one of three comparable experiments.