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. Author manuscript; available in PMC: 2017 Aug 25.
Published in final edited form as: Cell. 2016 Aug 25;166(5):1117–1131.e14. doi: 10.1016/j.cell.2016.07.032

Figure 7. Inhibition of PHD proteins improves adoptive cell transfer immunotherapy.

Figure 7

(A) Experimental schema of adoptive cell transfer immunotherapy (ACT). Pre-transfer levels of T-bet and Foxp3 expression is shown.

(B) IFN-γ ELISA from culture supernatants of TRP-1 splenocytes stimulated in vitro ± DMOG.

(C–D) Pulmonary tumor burden in mice receiving ACT of indicated cells. Representative H&E (C) and total lung tumor metastatic nodules (D) are shown. Black arrows indicate representative area of tumor burden.

(E–F) Subcutaneous tumor growth in mice receiving ACT of indicated cells. Tumor area (E) and overall survival (F) are shown. Survival significance was assessed by a Log-rank Mantel-Cox test.

Data are representative of ≥ 2 independent experiments with > 5 mice per group. Bars and error represent mean ±SEM of replicate measurements. **P<0.01, ****P<0.0001 (Student’s t-test).

See also Figure S6.