Figure 2.
Expression of cx43 and evx1 during the joint initiation timeline. Qualitative changes in cx43 and evx1 gene expression are shown. Whole mount in situ hybridization for cx43 is shown at 72 hpf (A), 87 hpf (B), 96 hpf (C). Cryo-in situ hybridization for cx43 is shown at 72 hpa (A′), 87 hpf (B′), 96 hpf (C′). Whole mount in situ hybridization for evx1 is shown at 72 hpf (D), 87 hpf (E), 96 hpf (F). Cryo-in situ hybridization for evx1 is shown at 72 hpa (D′), 87 hpf (E′), 96 hpf (F′). Asterisks identify regions of gene expression. Note that cx43 mRNA is detected medially in the dermal fibroblasts and laterally in a subset of cells in the osteoblast lineage, while evx1 mRNA is restricted to a subset of cells in the osteoblast lineage.
