Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Dev Dyn. 2017 Jul 12;246(9):691–699. doi: 10.1002/dvdy.24531

Figure 3.

Figure 3

Increased cx43 at the time of joint formation reduces the frequency of evx1-positive rays. (A) Whole mount ISH shows increased cx43 and decreased evx1 expression at the time of joint initiation (87 hpa) in the Tg(hsp70:miR-133sp)pd48 line following a heat pulse at 72 hpa. Transgene-positive fins, sp(+) (n = 9 fins), were compared to non-transgenic sp(-) siblings (n = 7 fins). (B) The sp(+) transgenic fish showed reduced frequency of evx1 expression compared to the sp(-) non-transgenic sibling. Both sp(+) and sp(-) fish were similarly treated for heat shock. Statistically significant differences were determined by the student’s t-test where p < 0.01 (**). Error bars represent the standard deviation.