Figure 5.
Inhibition of calcineurin (FK506-treatment) increases cx43 and reduces the frequency of evx1-positive rays. (A) Whole Mount ISH shows increased cx43 and decreased evx1 expression at the time of joint initiation (87 hpa) in FK506-treated fish versus DMSO (negative control). (B) FK506-treated fish (n= 3 fins) showed reduced frequency of evx1 positive fin rays compared to DMSO-treated fish (n = 4 fins). Statistically significant differences were determined by the student’s t-test where p < 0.01 (**). Error bars represent the standard deviation.
