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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Dev Dyn. 2017 Jul 12;246(9):691–699. doi: 10.1002/dvdy.24531

Figure 6.

Figure 6

Inhibition of calcineurin (FK506-treatment) influences joint formation and regenerate length. (A, B) Calcein staining detects bone matrix and reveals joint failure and increased regenerate length in FK506-treated wild-type fish compared to control DMSO-treated wild-type fish. Panels on the left show fluorescence, while panels on the right show fluorescence plus bright field to better illustrate the end of the regenerating fin (the dotted line indicates the amputation plane). Arrowheads point to joints. Double-headed arrows identify regenerate length. (C) Regenerate length is significantly increased FK506-treated (n = 18) versus DMSO-treated (n = 18, negative control) fish. Statistically significant differences were determined by the student’s t-test where p < 0.001 (***). Error bars represent the standard deviation.