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. Author manuscript; available in PMC: 2018 Oct 1.

Published in final edited form as: Int J Biol Macromol. 2017 May 22;103:965–971. doi: 10.1016/j.ijbiomac.2017.05.119

Fig. 3 — The oligomeric states of TBC1D4 RabGAP (A), CTR (B), and RabGAP-CTR1 (C) were analyzed by sedimentation equilibrium. (A) Sedimentation equilibrium distributions of RabGAP (TBC1D4 865-1191) measured at 18,000 rpm and 280 nm (□) are fitted for both an ideal monomer (solid line, 1×) and a dimer (dotted line, 2×). The random residual distributions shown for a monomer (□, 1×) in comparison to a dimer (○, 2×) indicate that RabGAP exists as homogeneous monomers in solution (inset). (B) Sedimentation equilibrium distributions of CTR (TBC1D4 1192-1299) measured at 18,000 rpm and 280 nm (□) are fitted for an ideal dimer (solid line, 2×), monomer, and a trimer (dotted line, 1×, 3×). Random residual distributions shown for a dimer (●, 2×) in comparison to either a monomer (△, 1×), or a trimer (▽, 3×) indicate that CTR exists as homogeneous dimers in solution (inset). (C) Sedimentation equilibrium distributions of RabGAP-CTR1 (TBC1D4 865-1299) measured at 13,000 rpm and 230 nm (○) are fitted for an ideal dimer (solid line, 2×), monomer, and a trimer (dotted line, 1×, 3×). Random residual distributions shown for a dimer (●, 2×) in comparison to either a monomer (△, 1×), or a trimer (▽, 3×) indicate that RabGAP-CTR1 exists as homogeneous dimers in solution (inset).

Fig. 3 — The oligomeric states of TBC1D4 RabGAP (A), CTR (B), and RabGAP-CTR1 (C) were analyzed by sedimentation equilibrium. (A) Sedimentation equilibrium distributions of RabGAP (TBC1D4 865-1191) measured at 18,000 rpm and 280 nm (□) are fitted for both an ideal monomer (solid line, 1×) and a dimer (dotted line, 2×). The random residual distributions shown for a monomer (□, 1×) in comparison to a dimer (○, 2×) indicate that RabGAP exists as homogeneous monomers in solution (inset). (B) Sedimentation equilibrium distributions of CTR (TBC1D4 1192-1299) measured at 18,000 rpm and 280 nm (□) are fitted for an ideal dimer (solid line, 2×), monomer, and a trimer (dotted line, 1×, 3×). Random residual distributions shown for a dimer (●, 2×) in comparison to either a monomer (△, 1×), or a trimer (▽, 3×) indicate that CTR exists as homogeneous dimers in solution (inset). (C) Sedimentation equilibrium distributions of RabGAP-CTR1 (TBC1D4 865-1299) measured at 13,000 rpm and 230 nm (○) are fitted for an ideal dimer (solid line, 2×), monomer, and a trimer (dotted line, 1×, 3×). Random residual distributions shown for a dimer (●, 2×) in comparison to either a monomer (△, 1×), or a trimer (▽, 3×) indicate that RabGAP-CTR1 exists as homogeneous dimers in solution (inset).