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. 2017 Aug 8;7:7599. doi: 10.1038/s41598-017-07953-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAPE and CAPE-pNO₂ induce cell cycle arrest at the G0/G1 phase in colon cancer cells. The percentage of cells in G0/G1, S and G2/M phase was calculated using Multicycle software. Changes in the cell cycle distribution in HT-29 (A) and HCT-116 (B) cells after treatment for 48 h with the concentration from 0 to 40 μmol/L. HT-29 (C) and HCT-116 (D) cells were stained with PI and were analysed by flow cytometry. The data represented the means ± SD from three independent experiments, and error bars represented with STDEV (SD). *p < 0.05, **p < 0.01: CAPE and CAPE-pNO₂ compared with the control. ^# p < 0.05, ^## p < 0.01: CAPE-pNO₂ compared with CAPE at the same concentration.