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. 2017 Aug 8;7:7599. doi: 10.1038/s41598-017-07953-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of apoptosis-related and cycle-related proteins by CAPE and CAPE-pNO₂ treatment in HT-29 cells. HT-29 cells were treated with CAPE and CAPE-pNO₂ at a dose of 0, 10, 20 or 40 μmol/L for 48 h. (A) Apoptosis-related proteins in the P53 signalling pathway such as Bax, Pro-Caspase-3, Cleaved-Caspase-3, CytoC and P38 were determined by western blotting. (B) The cell cycle-related protein expression levels of P21 ^Cip1, P27^Kip1, P53, CDK2 and c-Myc were determined by western blotting. The blots were representative of three independent experiments. Data represented the means ± SD from three independent experiments, and error bars represented the STDEV (SD). *p < 0.05, **p < 0.01: CAPE and CAPE-pNO₂ compared with the control. ^# p < 0.05, ^## p < 0.01: CAPE-pNO₂ compared with CAPE at the same concentration.