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. 2017 Aug 8;7:7555. doi: 10.1038/s41598-017-08085-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SG2NA downregulation increases the G1 phase duration while upregulation increases G2 phase duration. (A) Cells stably overexpressing 78 kDa and (B) underexpressing SG2NA were harvested, 100 μg of cell lysates were loaded on SDS-polyacrylamide gel and immunoblotted with SG2NA and β-actin antibody. Numbers on the top of bands represent fold change in protein levels relative to untransfected NIH3T3 cells. (C) Representative FACS histograms of NIH3T3 cells stably transfected with vector alone, 78 kDa SG2NA and shRNA against SG2NA. (D) Graphical representation of the percentage of cells present in different phases of cell cycle during the cell cycle preogression. (E) Left panel: Lysates of normal NIH3T3 cells and SG2NA downregulated cells were loaded on SDS-polyacrylamide gel and probed with cyclin D1 and β-actin. Right panel: Graph representing real time analysis by qPCR for cyclin D1 mRNA expression in normal NIH3T3 cells and SG2NA downregulated cells. Error bars are the standard deviation of 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Full length blots for panel (A) and (B) are shown in Supplementary Figure S1.