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. 2017 Aug 8;7:7571. doi: 10.1038/s41598-017-07297-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of interested genes in Bryum argenteum. Selected transcript abundance profiles were validated using quantitative real-time PCR (qPCR), including genes encoding ERF transcription factor (a), heat shock factor (HSF) (b), MYB transcription factor (c), heat shock protein 70 (d), dehydrin (e), late embryogenesis abundant (LEA)-like protein (f), sucrose synthase (g), early light induced protein (ELIP) (h), photosystem I light harvesting complex gene 2 (LHCA2) (i), protein kinase (SNRK) (j) and zeaxanthin epoxidase (ZEP) (j). qPCR quantitative gene expression data were shown as the mean ± SEM. Correlation of the expression levels evaluated using qPCR and RNA-Seq was statistically assessed by the calculation of Pearson correlation coefficient (r) and P-value were also indicated (**P-value < 0.005, *P-value < 0.05, ns: not significant or P-value > 0.05).