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. 2017 Aug 8;7:7483. doi: 10.1038/s41598-017-07756-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of amiloride on NaCl (150 mM), Ala-Arg (AR; 50 μM), and the mixture of NaCl and AR in ENaCα (a), ENaCδ (b), and NHE1 (c) siRNA-transfected HBO cells. Responding cells were measured by intracellular Ca²⁺ changes. The concentration of amiloride was 50 μM. Each experiment was performed seventeen times. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant.