Figure 5.

Confocal microscopy of GFP expressing P aeruginosa biofilm on a metal washer support SEM findings that AMF initially targets EPS. Confocal microscopy images of infected washers exposed to AMF show a decrease in the biofilm matrix (red) and number of bacteria (green) as the duration of an AMF exposure was increased. For exposures greater than 3 minutes it was difficult to detect any bacteria on the washer surface. At 1 minute, the number of bacteria appear relatively unaffected, but the biofilm matrix signal is greatly decreased, suggesting heating initially affects this matrix. The washers were incubated in GFP-PA01 for 18 hours to form a biofilm. The scale bars in each panel indicate 20 μm.