Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 8;7:7511. doi: 10.1038/s41598-017-07903-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TRPC6 deletion attenuates pressure overload-induced cardiac fibrosis but not fibrotic gene expression in mice. (a) Heart weight (HW) /body weight (BW) ratio in WT and TRPC6^(−/−) mice 6 weeks after TAC. WT- TAC(−) (sham) (n = 6), TRPC6^(−/−)-sham (n = 6), WT-TAC (n = 13), TRPC6^(−/−)-TAC (n = 11). (b) Representative images of wheat germ agglutinin (WGA) staining for cross-sectional areas (CSA) measurement 6 weeks after TAC (left). Green; WGA, blue; DAPI. Quantitative results are shown in right panel. WT-sham (n = 6), TRPC6^(−/−)-sham (n = 6), WT-TAC (n = 13), TRPC6^(−/−)-TAC (n = 11). (c,d) TRPC6 contributes to TAC induced cardiac fibrosis. (c) Representative images of picrosirius red staining 6 weeks after TAC and results of interstitial fibrosis 6 weeks after TAC. WT-sham (n = 6), TRPC6^(−/−)-sham (n = 6), WT-TAC (n = 13), TRPC6^(−/−)-TAC (n = 11). (d) Relationship between fibrosis and hypertrophy 6 weeks after TAC. WT-sham (n = 6), TRPC6^(−/−)-sham (n = 6), WT-TAC (n = 13), TRPC6^(−/−)-TAC (n = 11). (e) Absence of TRPC6 does not affect pressure overload-induced LV dysfunction. LV end-diastolic pressure (LVEDP; left), LV end-systolic pressure (LVESP; middle) and LV dP/dt_max (right) in mice 6 weeks after TAC. WT-sham (n = 6), TRPC6^(−/−)-sham (n = 6), WT-TAC (n = 13), TRPC6^(−/−)-TAC (n = 11). (f) Expression levels of hypertrophy-related and fibrosis-related mRNAs in mouse hearts 1 week after TAC. WT-sham (n = 3), other groups (n = 4 each). Error bars, s.e.m. *P < 0.05, **P < 0.01. Results of WT mice were the same as those reported previously^{17, 18}.