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. 2017 Aug 8;7:7511. doi: 10.1038/s41598-017-07903-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Involvement of TRPC6 in TRPC3/Nox2-mediated ROS production. (a) Expression levels of TRPC3 and TRPC6 mRNAs in NRCMs cultured in low (LG, 5.5 mM) or high (HG, 25 mM) glucose-containing medium (n = 3 each). (b) Expression levels of TNFα or IL1β in NRCMs cultured in LG or HG medium (n = 7 each). (c) Expression levels of TRPC3 and TRPC6 mRNAs in NRCMs transfected with siRNAs. (n = 5 each). (d,e) Abundance of Nox2 protein (d) and ROS production (e) in siRNA-transfected NRCMs cultured in LG or HG medium (n = 3 each). Full-length blots are presented in Supplementary Fig. 4. (f) Ca²⁺ responses induced by hypotonic stress (70 mM NaCl) in siRNA-transfected NRCMs. Averaged time courses (left) and [Ca²⁺]_i increases shown as AUC (right) (n = 45–46 cells). Error bars, s.e.m.^*P < 0.05, ^**P < 0.01.