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Primer sequences used for the segregation analysis of the two novel MCDR3 duplications identified in the study.
| Duplication size | Primer sequence | Tm (°C) | Length (bp) | |
|---|---|---|---|---|
| 43 kb | F | 5′-TTGTGGACTGAGCAAGCAAG-3′ | 63 | 532 |
| R | 5′-GGAGCAGAAGTTAAATGTGGAGA-3′ | |||
| 45 kb | F | 5′-TTTGCTTGATCAATTCTGCTG-3′ | 63 | 500 |
| R | 5′-TTCTCAGTTGGAAGAGCACAAA-3′ | |||
Tm = Temperature of melting.
