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. 2017 Aug 8;7:7536. doi: 10.1038/s41598-017-08000-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rejection of allogeneic erythrocytes in rag1 ^+/− and rag1 ^−/− fish. Flow cytometry histograms show the transferred positive cells in rag1 ^+/− (left) or rag1 ^−/− recipients (right) (A). Horizontal bars in the histogram indicate the 5(6)-carboxyfluorescein diacetate N-succinimidyl ester-positive region. The data are representative of three independent samples. The percentage of variable transferred cells in recipients is shown in non-immunized and immunized fish (B). Asterisk (*) indicates a significant difference between the two strains (p < 0.05).