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. 2017 Aug 8;7:7554. doi: 10.1038/s41598-017-06211-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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QKI proteins regulate self-splicing in mice. (a) A schematic of the 3′-UTR of mouse qkI gene. Arrows indicate location of primers used for splicing analysis. Primer 1 is in exon 6 that is common to all QKI isoforms. (b) (Left) RT-PCR analysis using primers #1 and #2 from n = 3 mice brain RNA/genotype. The lower band corresponds to qkI-7 isoform (exon 6; 7a) whereas the upper band corresponds to the unspliced pre-mRNA. (Right) The pixel densities of PCR bands from 3 independent mice brain RNA samples were quantified using ImageJ software, normalized over constitutive exon, and then normalized over wild-type mice represented as mean ± SEM (Student t-test, **p < 0.001). (c) (Left) RT-PCR analysis using primers #1 and #3 from n = 3 mice brain RNA/genotype. The lower band corresponds to qkI-6 isoform (exon 6; 7b) whereas the upper band corresponds to qkI-7 3′-UTR. (Right) The pixel densities of PCR bands from 3 independent mice brain RNA samples were quantified using ImageJ software, normalized over constitutive exon and then normalized over wild-type mice and represented as mean ± SEM (Student t-test, *p < 0.05). (d) (Left) RT-PCR analysis using primers #1 and #4 from n = 3 mice brain RNA/genotype. The lower band corresponds to qkI-5 isoform (exon 6; 7c) whereas the upper band corresponds to qkI-6 3′-UTR. (Right) The pixel densities of PCR bands from 3 independent mice brain RNA samples were quantified using ImageJ software, normalized over constitutive exon and then normalized over wild-type mice and represented as mean ± SEM (Student t-test, *p < 0.05, **p > 0.01). (e) (Left) RT-PCR analysis using primers #1 and #5 (qkI-5 isoform: exon 6; 7c; exon8), primers #1 and #6 (qkI-5 isoform: exon 6; 7c; exon8; 3′-UTR), constitutive qkI exon 1, and GAPDH from n = 3 mice brain RNA/genotype. (Right) The pixel densities of PCR bands from 3 independent mice brain RNA samples were quantified using ImageJ software, normalized over constitutive exon and then normalized over wild-type mice and represented as mean ± SEM (Student t-test, *p < 0.05).