Figure 6.
Both sodium oleate and sodium linoleate induces a regulatory phenotype in bone marrow-derived myeloid cells. Bone marrow cells isolated from naïve mice were cultured with GM-CSF (40 ng/ml) and FFA as indicated for 7 days. FFA-treated cells or controls were co-cultured with CD4+ T cells in the ratios as indicated. Cell proliferation was evaluated by CFSE staining after 72 h (A/E). Supernatants were served for NO analysis (B/F) and IFNγ detection (C). Shown is the mean ± SD from two to four independent experiments. *p < 0.05; **p ≤ 0.01;***p ≤ 0.001.