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. 2017 Aug 8;7:7603. doi: 10.1038/s41598-017-07855-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myo10-deficient osteoclasts undergo less pSMAD1, 5, 8 activation. Western blot of osteoclast extracts that were cultured in the presence of M-CSF and RANKL for 3 days. (A) MYO10, NFATc1, and pSMAD 1/5/8 levels were analyzed by Western blot. Actin was analyzed as a loading control. (B) pERK, ERK, p-p38 and p38 levels were analyzed by Western blot. Western blots were cropped to show only relevant bands. Full length western blots are presented in Supplemental Fig. 4.