Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 8;7:7514. doi: 10.1038/s41598-017-06569-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of histone methylation in Kdm4b-EGFP expressing MEFs. (a) Immunofluorescence of histone modifications in non-induced (NI) vs induced (I) MEFs. Cells were co-stained for EGFP and histone modification antibodies. Arrowheads and arrows indicate EGFP-positive and –negative nuclei, respectively. (b) Quantification of global histone methylation in NI vs I MEFs. Values represent normalized pixel intensity ± SEM. Asterisks indicate significant differences. N = number of nuclei quantified, RU = relative units; ***P < 0.001; **P < 0.01. (c,d) Kdm4b-dependent changes in levels of H3K9 methylation states. (c) Histone extracts from NI vs I MEFs were quantified for the indicated histone modifications by western blot. Shown are cropped representative blots for the different methyl states of H3 and a cropped representative Ponceau staining. (d) Quantification of western analysis. Results represent H3 methylation levels in induced cells as a percentage of non-induced levels ± SEM. Dotted line indicates NI level; n = number of replicate experiments, Bars with an asterisk differ from NI level at **P < 0.005, *P < 0.05, as determined by two–tailed paired t-test on normalised band intensities.