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. 2017 Aug 8;7:7514. doi: 10.1038/s41598-017-06569-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H3K9/36me3 levels in NT reconstructs generated from induced vs non-induced Kdm4b MEFs. (a) NT reconstructs were fixed and co-stained for DNA (H33342) and antibodies specific for histone modifications. Cells were analyzed 10 minutes (upper), 1 h (middle) and 8 h (lower panel) after transfer of donor DNA (D) into cytoplast (C); One-cell reconstructs were fixed and stained with H33342 (DNA) and antibodies specific for H3K9me3 and H3K36me3. PPB = pseudo polar body, PPN = pseudo-pronucleus. (b) Quantification of immunofluorescence analysis in (a). Values represent normalized pixel intensity ± SEM. N = number of NT reconstructs quantified. Different letters indicate significant differences between MEFs from the same groups (induced or non-induced) at different time points, ***P < 0.0001, **P < 0.01, *P < 0.05 by two-tailed unpaired t-tests.