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. 2017 Aug 8;7:7595. doi: 10.1038/s41598-017-07746-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of synaptic components in type III taste cells. Immunostaining was used to identify synaptic components PCLO and CPLX2 (red, a,d) and the type III marker 5-HT (green, b,e). Merged images (c,f) indicate that PCLO is expressed in almost all 5-HT expressing cells in addition to several non 5-HT expressing cells while CPLX2 is expressed exclusively in almost all 5-HT expressing cells. Arrows point to cells co-expressing 5-HT and PCLO or CPLX2. Scale bars: C = 20 µm, F = 40 µm.