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. 2017 Aug 8;7:7595. doi: 10.1038/s41598-017-07746-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of semaphorin pathway genes in Tas1r3+ taste cells. Immunostaining identifies the semaphorin pathway components PLEXINB1, CRMP2, FES and SEMA4A (red, a,d,g,j). Intrinsic fluorescence of Tas1r3-GFP identifies Tas1r3+ taste cells (green, b,e,h,k). Merged images (c,f,i,l) indicate that most Tas1r3+ cells express the semaphorin pathway components examined, and that they are expressed in other taste cells also. Arrows point to cells co-expressing Tas1r3-GFP and PLEXINB1, CRMP2, FES or SEMA4A. Scale bars: c = 16 µm, f, i, l = 40 µm.