Figure 5.

Heteronuclear NOE and RDC Data for FBN1^E2cbEGF1

(A) {¹H}-¹⁵N heteronuclear NOE ratios are plotted as a function of sequence for FBN1^E2cbEGF1. Low heteronuclear NOE ratios (<0.5) at the N terminus of EGF2 and for two loops in Hyb1 (†, ^∗) indicate significant flexibility on a picosecond to nanocsecond timescale in these regions. The residues linking EGF3 to Hyb1, indicated by a thick black line, show NOE ratios characteristic of a rigid protein backbone demonstrating that the EGF3-Hyb1 linker is not flexible on a fast timescale. Error bars for the NOE ratio were estimated from 500 Monte Carlo simulations using baseline noise as a measure of the error in the peak heights.

(B) Distribution of RDC values measured in 4% bicelles for the EGF2-EGF3 and Hyb1-cbEGF1 domain pairs of FBN1^E2cbEGF1. The different distribution of RDC values for the two pairs suggests that there may be some slower timescale flexibility at the EGF3-Hyb1 interface. Similar differences in RDC distribution are observed for RDCs measured in 2.2% C12E6/n-hexanol (data not shown).