Table 1.

List of primers and sequences

Exon #	Forward primer (5’-3’)	Reverse primer (5’- 3’)
1	CGCTCCTCTCTGAGACAGAC	TTTTATAGAACATGCAACATTATCC
2.1	AATGAGTTTGGTTGAGGCAG	ATATCCAGCAGGGCAGATG
2.2	CAGTGGGACAATCTGTGAAAC	AATGTCACCTCTGCTTCTGC
3	GCTAAATTATGAACACTTTGCTAAAAC	GGTAAAATAGTTCATGGTCAGGG
4	CATGGGTCTTGGGTTGATAG	TTCATTTCATTTGCTATAAGCG
5	AACCTCCTTTTAGGCAAATG	GGTTAAAGCCATGGTCTGC
6.1	GAGCTATTCATGCACTTCTGC	GCCTCTGCAAATATTACCTCC
6.2	GAAGCTGGAGCTGCTAAGTG	TTTGCTGTTTCTGCTCTGC
7.1	TCCATCCCTTCTGTCTTTTG	TCCTAGGTTTTGTGAAGACTGA
7.2	TGGTGGGTCAGTAACATCATC	GCAATGCTGACTCCAAACTC
8	CAGATATGTGGTTTCACCGTC	TCTGTGTTTGCTCTTGGAAC
9.1	AAAAGCAACTAGCACAGTATGTAAC	AACTGCAAACAGCCAGTGAC
9.2	TGTGGGAGACAGAGCTATTGA	CTTGAGGAGAGAGCTTTCCAA
10	CTTTTCTTGAATGAGATGAACAAG	GAACTTTGAGTAATCCCATCATTC
11	GCTGTTCCAGAGAGATAAGGC	CTCAACAACTGGCTCGTCAT
12	TTCCTGAGTAGTTCCATTGTCC	CCCAGTTGCAGATTAACATTG

List of primer used for Sanger sequencing of CRB1 gene. Multiple overlapping primers were used to amplify and sequence exons 2, 6, 7, and 9.