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. 2017 Sep;21(5):294–302. doi: 10.18869/acadpub.ibj.21.5.294

Table 1.

List of primers and sequences

Exon # Forward primer (5’-3’) Reverse primer (5’- 3’)
1 CGCTCCTCTCTGAGACAGAC TTTTATAGAACATGCAACATTATCC
2.1 AATGAGTTTGGTTGAGGCAG ATATCCAGCAGGGCAGATG
2.2 CAGTGGGACAATCTGTGAAAC AATGTCACCTCTGCTTCTGC
3 GCTAAATTATGAACACTTTGCTAAAAC GGTAAAATAGTTCATGGTCAGGG
4 CATGGGTCTTGGGTTGATAG TTCATTTCATTTGCTATAAGCG
5 AACCTCCTTTTAGGCAAATG GGTTAAAGCCATGGTCTGC
6.1 GAGCTATTCATGCACTTCTGC GCCTCTGCAAATATTACCTCC
6.2 GAAGCTGGAGCTGCTAAGTG TTTGCTGTTTCTGCTCTGC
7.1 TCCATCCCTTCTGTCTTTTG TCCTAGGTTTTGTGAAGACTGA
7.2 TGGTGGGTCAGTAACATCATC GCAATGCTGACTCCAAACTC
8 CAGATATGTGGTTTCACCGTC TCTGTGTTTGCTCTTGGAAC
9.1 AAAAGCAACTAGCACAGTATGTAAC AACTGCAAACAGCCAGTGAC
9.2 TGTGGGAGACAGAGCTATTGA CTTGAGGAGAGAGCTTTCCAA
10 CTTTTCTTGAATGAGATGAACAAG GAACTTTGAGTAATCCCATCATTC
11 GCTGTTCCAGAGAGATAAGGC CTCAACAACTGGCTCGTCAT
12 TTCCTGAGTAGTTCCATTGTCC CCCAGTTGCAGATTAACATTG

List of primer used for Sanger sequencing of CRB1 gene. Multiple overlapping primers were used to amplify and sequence exons 2, 6, 7, and 9.