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Primer sequences, amplicon sizes, and the annealing temperature in quantitative real-time PCR
| Gene | Sequence of primers 5’→3’ | Size (bp) | An. Temp. (°C) |
|---|---|---|---|
| TGF-β1 | F: CGACTACTACGCCAAGGAGGT’ | 149 | 62 |
| R: AGAGCAACACGGGTTCAGGTA | |||
| Endoglin | F: AGGCGGTGGTCAATATCC | 109 | 62 |
| R: AAGTGTGGGCTGAGGTAG | |||
| β-actin | F: GCCTTTGCCGATCCGC | 90 | 58 |
| R: GCCGTAGCCGTTGTCG |
An.Temp., annealing temperature; F, forward primer; R, reverse primer
