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. 2017 Jul 17;38(3):1420–1430. doi: 10.3892/or.2017.5826

Figure 2.

Figure 2.

Effects of cisplatin on tumorsphere formation in vitro. The tumorsphere formation assay was used to analyze the effects of cisplatin (0, 0.5, 1, 5, 10 and 50 µM) on the stemness of HCT116 and LoVo cells. (A) Representative image of tumorspheres obtained through microscopy. (B) Histogram of the number of tumorspheres. (C) Histogram of the mean tumorsphere diameter per well. (D) Representative image of the colony formation assay obtained through microscopy. (E) Histogram of the number of colonies. ***p<0.001, **p<0.01, *p<0.05.