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. 2017 Jul 17;38(3):1420–1430. doi: 10.3892/or.2017.5826

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Bufalin is effective against cisplatin with regard to the stemness of colorectal cancer cells in vitro. The cells derived from PTSs treated with cisplatin (5 µM), referred to as PTSCs^cis, are used for the secondary tumorsphere formation assay. These cells were treated with control (Con), 5 µM cisplatin, 5 nM bufalin, and combination (Com) separately. (A) Representative image of secondary tumorspheres. (B) Histogram of the number of secondary tumorspheres. (C) Histogram of secondary tumorsphere diameters. (D) SP ratio assay of STSCs using flow cytometry. (E) Histogram of the SP ratio. (F) Representative image of Hoechst 33342 staining of STSCs. (G) Histogram of the ratio of cells with negative staining for Hoechst 33342. ***p<0.001, **p<0.01, *p<0.05.