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Bufalin synergistically inhibits the proliferation of colorectal cancer cells with cisplatin and decreases stemness in vivo. A subcutaneous xenograft model of HCT116 cells was treated with cisplatin alone or cisplatin + bufalin for 3 weeks. Tumors were photographed (A) and weighed (B). (C) Hematoxylin and eosin staining and immunohistochemistry were performed to evaluate the expression of stemness markers.
