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. 2017 Jul 18;38(3):1473–1481. doi: 10.3892/or.2017.5831

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Copyright: © Ji et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — miR-374 is overexpressed in RGI-MSCs and mediates their increased proliferation and migration abilities. (A) The expression of miRNAs (miR-17-5p, miR-99a, miR-221 and miR-374) in the serum of normal and MNNG-induced rats and in RGN-MSCs and RGI-MSCs; *P<0.05. (B) The expression of miR-374 in RGN-MSCs transfected with control and miR-374 mimics; ***P<0.001. (C) Cell counting assays for RGN-MSCs transfected with control and miR-374 mimics at the indicated time points; ***P<0.001. (D) Transwell migration assays for RGN-MSCs transfected with control and miR-374 mimics. Original magnification of the left panel, ×100; the right panel, ×200; scale bar, 50 µm; ***P<0.001. (E) Expression of miR-374 in RGI-MSCs with control and miR-374 inhibitor; ***P<0.001. (F) Cell counting assays for RGI-MSCs transfected with control and miR-374 inhibitor at the indicated time points; **P<0.01. (G) Transwell migration assays for RGI-MSCs transfected with control and miR-374 inhibitor. Original magnification of the left panel, ×100; the right panel, ×200; scale bar, 50 µm; *P<0.05.