Fig.2.
Agonist competition for [3H]XAC binding in (A) solubilized bovine brain membranes and (B) XAC-Affi-Gel-purified preparation. Membrane solubilization, affinity chromatography and binding assays were performed as described in section 2. The final concentration of [3H]XAC was ~ 0.5 nM in both assays. These plots are representative of 3 similar experiments.