Table 1.
Prevalence of chromosome 17 aneusomy in invasive breast cancer estimated by FISH analysis
| Breast material | Number of specimens | Number of nuclei counted | Disomy | Monosomy | Polysomy | |||||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
||||||||
| Cutoff | % | Cutoff | % | Cutoff | % | Association with ERBB2 protein expression* | ||||
| Herrington et al (1995)29 | FNA | 49 | ≥100 | MS=2 | 40 | MS<2 | 5.0 | MS>2 | 55 | NR |
| Ichikawa et al (1996)30 | FNA | 80 | >200 | >80† | 48 | >15‡ | 14 | >20§ | 34 | NR |
| McManus et al (1999)31 | FNA | 69 | ≥100 | 2¶ | 26 | ≥20‡ | 0.0 | ≥10§ | 68 | NR |
| Tsukamoto et al (2001)32 | FNA | 113 | >100 | NR | 41 | >15‡ | 22 | >20§ | 37 | NR |
| Fehm et al (2002)33 | Touch prep | 74 | ≥100 | 2¶ | 41 | >15‡ | 11 | >6§ | 38 | NR |
| Nakopoulou et al (2002)34 | FFPE | 42 | 200–400 | >70† | 24 | >40‡ | 38 | >15§ | 38 | NR |
| Wang et al (2002)35 | FFPE | 189 | ≥60 | 1.76–2.25¶ | 49 | <1.76¶ | 2.6 | ≥2.26¶ | 49 | - |
| 2.26–3.75]∥ | 43 | - | ||||||||
| >3.76** | 5 | 3+†† | ||||||||
| Watters et al (2003)36 | FFPE | 214 | 60 | 1.35–1.85¶ | 46 | <1.35¶ | 7.5 | >1.86¶ | 47 | NR |
| Ma et al (2005)37 | FFPE | 893 | 60 | 1.5–2.25¶ | 49 | <1.5¶ | 8.9 | ≥2.26¶ | 42 | 3+ |
| 2.26–3.75∥ | 35 | - | ||||||||
| >3.76** | 7 | 3+ | ||||||||
| Merola et al (2006)38 | FFPE | 343 | 200 | 2¶ | 30 | 1¶ | 24 | >3¶ | 46 | 2+ |
| 2–4∥ | 40 | NR | ||||||||
| >4** | 5.8 | NR | ||||||||
| Takehisa et al (2007)39 | FNA | 40 | >100 | NR | 48 | >15‡ | 10 | >20§ | 43 | NR |
| Hofmann et al (2007)25 | FFPE | 95 | 60 | NR | NR | NR | NR | ≥3¶ | 27 | 3+ |
| Hyun et al (2008)40 | FFPE | 309 | ≥60 | 1.25–2.25¶ | NR | <1.25¶ | 1.3 | ≥2.26¶ | 32 | 3+ |
| 2.26–3.75∥ | 26 | NR | ||||||||
| >3.76** | 5.8 | NR | ||||||||
FNA=fine needle aspirate. FFPE=formalin-fixed paraffin-embedded. NR=not explicitly reported in reference. MS=mode chromosome 17 signal per nucleus. ..=no association found.
The association between polysomy 17 and ERBB2 expression (scored as 0–3+ staining intensity) in ERBB2 non-amplified tumours.
Percent of cells displaying 2 signals per nucleus.
Percent of nuclei with loss of centromeric region or entire chromosome (typically with 0 or 1 chromosome 17 signal per nucleus).
Percent of nuclei with gain of centromeric region or entire chromosome (typically >2 or ≥3 chromosome 17 signals per nucleus).
Average number of chromosome 17 signals per nucleus.
Low-level polysomy.
High-level polysomy.
Association was found between high polysomy (>3.76) and 10 tumours with ERBB2 immunohistochemical scores of 3+.