Table 1.
Primers used for real-time PCR validation
| target gene | forward primer (5′ - > 3′) | reverse primer(5′ - > 3′) |
|---|---|---|
| Chitotriosidase | TACTGCGATTGGCCATACAA | TGCCTGTGTAGTTCGAGGTG |
| Meprin A | GCTTGGGTACATCGACCACT | CTGCTTTGGTTCCAGTGTCA |
| Paramyosin | CCGAACTCGCAGAAAAGAAC | CGTAGAGCTTCCTCGGTGTC |
| Myosin, striated muscle | CTCTTGTTGCCCCAGGATTA | CTGGTAGCTCCACCAGCTTC |
| Acetylcholine receptor | GTCAAAGTCGGCCACTCACT | GTCTGACCGTCGTTGGTTCT |
| Glycine-rich secreted protein | CACACGGTCTTACTGGAGCA | GTTCGCCTTGTTCACCTTGT |
| Valine-rich secreted protein | AAAGTGCCATTCGAGACACC | GGGCTGGGAACTCTGAATTT |
| SCP domain containign protein | TCAAACACTGCGTCAAGACC | GGATCCACGTTTTCTCTTGC |
| Serine protease inhibitor | AGGCCAACTGCAAAAACG | CCGTCAACTCCACACACG |
| Tyrosinase-like protein 2 | CAGAGCCCTACCTCCAGATG | TGACTGCTCGCTTTGTATGG |
| EF1alpha | CTCTTCGTCTCCCACTCCAG | ACCAGGGAGAGCTTCAGTCA |
| Ribosomal protein S2 | GCCATTGCCAATACCTATGC | GCCTGGTTGACGAGTATGGT |