Table 4.
The 25 most highly expressed genes in Mytilisepta virgata foot and representative significantly over-represented annotations by gene set enrichment analysis
| Most expressed genes | Over-represented annotations | |||
|---|---|---|---|---|
| Annotation | TPM | Term | Class | p-value |
| Byssal cuticle protein | 45,262.97 | Collagen triple helix repeat (20 copies) | PFAM | 0.00 |
| Byssal cuticle protein | 43,379.14 | Serine-type endopeptidase inhibitor activity | MF | 0.00 |
| Collagen-like protein | 42,699.96 | Negative regulation of coagulation | BP | 1.11E-16 |
| Byssal cuticle protein | 34,293.81 | Common central domain of tyrosinase | PFAM | 3.33E-16 |
| Collagen-like protein | 33,886.43 | Extracellular region | CC | 3.33E-16 |
| Serine protease inhibitor | 30,056.14 | Negative regulation of endopeptidase activity | BP | 6.77E-15 |
| Low complexity protein | 26,250.15 | Animal haem peroxidase | PFAM | 6.77E-14 |
| Collagen-like protein | 17,017.56 | Kazal-type serine protease inhibitor domain | PFAM | 4.35E-12 |
| YGH-rich protein-2 | 16,065.78 | Peroxidase activity | MF | 5.69E-12 |
| Collagen-like protein | 15,351.63 | Proteinaceous extracellular matrix | CC | 6.47E-12 |
| Collagen-like protein | 13,389.57 | Tissue inhibitor of metalloproteinase | PFAM | 6.83E-10 |
| Byssal cuticle protein | 12,178.71 | Collagen trimer | CC | 2.14E-8 |
| Collagen-like protein | 10,965.75 | Response to oxidative stress | BP | 5.45E-8 |
| Elongation factor 1 alpha | 10,722.32 | Regulation of keratinocyte differentiation | BP | 8.93E-8 |
| Collagen-like protein | 10,461.88 | Hydrogen peroxide catabolic process | BP | 1.05E-7 |
| YGH-rich protein-1 | 10,019.89 | Transforming growth factor beta binding | MF | 1.63E-7 |
| Precollagen NG | 9810.75 | Metal ion binding | MF | 8.77E-7 |
| YGH-rich protein-1 | 8119.26 | Extracellular space | CC | 9.20E-7 |
| Serine protease inhibitor | 7406.30 | Heme binding | MF | 2.55E-6 |
| Serine protease inhibitor | 7362.32 | Oxidoreductase activity | MF | 6.45E-6 |
| Collagen-like protein | 7349.68 | WAP-type ‘four-disulfide core’ | PFAM | 1.01E-5 |
| Collagen-like protein | 7307.02 | Quinone binding | MF | 1.26E-5 |
| Unknown | 7104.95 | Human growth factor-like EGF | PFAM | 1.78E-5 |
| Kazal-type serine proteinase inhibitor | 7095.67 | EGF-like domain | PFAM | 6.84E-5 |
| Low complexity protein | 6445.09 | Kunitz/Bovine pancreatic trypsin inhibitor domain | PFAM | 7.62E-5 |
See the complete list in Additional file 4
TPM Transcript Per Million, PFAM Pfam conserved domains, BP Gene Ontology Biological Process, MF Gene Ontology Molecular Function, CC Gene Ontology Cellular Component