Table 5.
The 25 most highly expressed genes in Mytilisepta virgata gills and representative significantly over-represented annotations by gene set enrichment analysis
| Most expressed genes | Over-represented annotations | |||
|---|---|---|---|---|
| Annotation | TPM | Term | Class | p-value |
| Alpha tubulin | 55,938.25 | Dynein heavy chain and region D6 of dynein motor | PFAM | 1.43E-13 |
| 40S ribosomal protein SA | 16,641.12 | Microtubule | CC | 0.00 |
| Elongation factor 1 alpha | 11,612.37 | Microtubule motor activity | MF | 6.20E-14 |
| Alpha tubulin | 8853.84 | Microtubule-based movement | BP | 2.52E-10 |
| Beta tubulin | 8710.17 | Motile cilium | CC | 3.06E-10 |
| Actin | 8589.14 | Axoneme assembly | BP | 1.57E-9 |
| Alpha tubulin | 8158.42 | Cilium movement | BP | 2.22E-9 |
| Low complexity glycine-rich protein | 7335.45 | Acetylcholine-activated cation-selective channel activity | MF | 5.94E-8 |
| Beta tubulin | 5932.28 | C1q domain | PFAM | 3.23E-7 |
| Actin | 5614.26 | ATP-binding dynein motor region D5 | PFAM | 1.32E-6 |
| Beta tubulin | 5144.94 | Tektin family | PFAM | 2.15E-6 |
| Beta tubulin | 5004.75 | Axonemal dynein complex | CC | 2.63E-6 |
| Actin | 4296.64 | Neurotransmitter-gated ion-channel ligand binding domain | PFAM | 6.18E-6 |
| 60s ribosomal protein P0 | 4296.41 | Dynein complex | CC | 1.06E-5 |
| non coding RNA | 4137.29 | ATPase activity | MF | 1.56E-5 |
| Ferritin | 3614.02 | Shisa/Wnt and FGF inhibitory regulator | PFAM | 3.71E-5 |
| Ribosomial Protein S10 | 3367.63 | Microtubule-binding stalk of dynein motor | PFAM | 4.65E-5 |
| Cyclophilin B | 3364.42 | Collagen trimer | CC | 5.40E-5 |
| Transcription factor ATF4 | 3351.91 | Immunoglobulin C1-set domain | PFAM | 7.45E-5 |
| Beta tubulin | 3296.13 | Fibrinogen beta and gamma chains, C-terminal globular domain | PFAM | 1.44E-4 |
| non coding RNA | 3201.67 | LITAF-like zinc ribbon domain | PFAM | 1.60E-4 |
| Cytochrome c oxidase polypeptide | 3059.53 | TIR domain | PFAM | 2.28E-4 |
| Outer dense fiber protein 3 | 3038.49 | Mitochondria-eating protein | PFAM | 2.42E-4 |
| Ribosomal Protein L15 | 2921.58 | Neurotransmitter-gated ion-channel transmembrane region | PFAM | 6.71E-4 |
| Ribosomal Protein S4 | 2769.43 | Cadherin domain | PFAM | 7.75E-4 |
See the complete list in Additional file 4
TPM Transcript Per Million, PFAM Pfam conserved domains, BP Gene Ontology Biological Process, MF Gene Ontology Molecular Function, CC Gene Ontology Cellular Component