Table 6.

The 25 most highly expressed genes in Mytilisepta virgata mantle rim and representative significantly over-represented annotations by gene set enrichment analysis

Most expressed genes		Over-represented annotations
Annotation	TPM	Term	Class	p-value
Actin	40,034.02	Proteinaceous extracellular matrix	CC	1.84E-7
Elongation factor 1 alpha	17,159.08	Extracellular region	CC	1.87E-7
alpha tubulin	16,259.80	Chitin binding Peritrophin-A domain	PFAM	4.58E-7
60s ribosomal protein L23	15,108.07	Matrixin	PFAM	1.14E-6
Actin	15,102.97	Calcium ion binding	MF	2.65E-6
40S ribosomal protein SA	11,849.66	WAP-type (Whey Acidic Protein) ‘four-disulfide core’	PFAM	3.65E-6
Actin	11,356.99	Extracellular matrix structural constituent	MF	3.66E-6
Alpha tubulin	8547.04	Immunoglobulin C1-set domain	PFAM	1.51E-5
Ferritin	7899.68	Serine-type endopeptidase inhibitor activity	MF	3.27E-5
Myosin	7382.76	Collagen catabolic process	BP	1.09E-4
low complexity protein - collagen-like	6579.48	Homeobox domain	PFAM	1.24E-4
60s ribosomal protein P0	5142.34	CD80-like C2-set immunoglobulin domain	PFAM	1.42E-4
Beta tubulin	4972.09	Metalloendopeptidase activity	MF	1.58E-4
Beta tubulin	4461.74	Cell junction	CC	8.89E-4
Superoxide Dismutase	4220.54	Cadherin domain	PFAM	9.51E-4
Actin	4066.75	Extracellular space	CC	1.77E-3
small heat shock protein 24.1	3933.60	Immunoglobulin V-set domain	PFAM	3.06E-3
Paramyosin	3898.32	Basement membrane	CC	3.57E-3
non coding RNA?	3796.60	Immunoglobulin I-set domain	PFAM	7.51E-3
non coding RNA?	3710.19	Integral component of membrane	CC	7.74E-3
Myosin	3690.69	/	/	/
non coding RNA?	3538.31	/	/	/
Cyclophilin	3464.80	/	/	/
Ribosomal protein S4	3459.95	/	/	/
Adenine nucleotide translocator	3431.98	/	/	/

See the complete list in Additional file 4

TPM Transcript Per Million, PFAM Pfam conserved domains, BP Gene Ontology Biological Process, MF Gene Ontology Molecular Function, CC Gene Ontology Cellular Component