Fig. 5.

RACK1 was a directly target of miR-155. a Relative luciferase activity of recombinant RACK1 promotor and U6 (control) vectors in BV2 cells which were co-transfected with scramble control or miR-155 mimic (n = 3). b Quantitative RT-PCR was performed to measure relative mRNA expression of RACK1 (n = 3), and (c) Western blot analysis was performed to measure the protein expressions of RACK1 in cells which were transfected with scramble, miR-155 mimic, inhibitor control, and miR-155 inhibitor. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; RT-PCR: reverse transcription polymerase chain reaction. *P < 0.05, ***P < 0.001