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. 2017 Jul 26;2017:3078194. doi: 10.1155/2017/3078194

Figure 2.

Figure 2

CCL20 secretion after Hst1 and LL-37 exposure. Skin or gingiva cells were exposed to either Hst1 or LL-37 for 24 hours, and CCL20 secretion was assessed by ELISA. (a) Keratinocyte monoculture, (b) fibroblast monoculture, and (c) keratinocyte-fibroblast coculture. (d) Keratinocyte-fibroblast coculture exposed to Hst1 and LL-37, together with either neutralizing antibodies to IL-1α (white bars) or isotype control (black bars). Each bar represents the mean ± standard error mean of 3 independent experiments each performed in duplicate, except that in (c) (N = 4). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.005.