Figure 1.

(a) Amplification of NAD kinase gene using NAD kinase 1 and NAD kinase 2 primers from the chromosomal DNA of Staphylococcus aureus. M Lane showing DNA ladder L1 lane showing amplified product of NAD kinase gene, (b) electrophoretogram showing the expression of recombinant NAD kinase clone in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Lane M: Molecular weight marker. Lane 1 and 2: Uninduced cell lysate of NAD kinase 1 clone. Lane 3: Induced cell lysate of NAD kinase 1 clone Lane 4: Purified recombinant NAD kinase. (c) Multiple sequence alignment of NAD kinase. (d) Phylogenetic tree based on NAD kinase sequence of Staphylococcus aureus with other bacterial NAD kinase sequences and Human NAD kinase