Figure 3. KOR activation does not affect OX1R calcium signaling.

CHO cells were transfected as indicated. After 48 h, cells were loaded with calcium-sensitive dye for 2 h. Cells were then treated with 1 μM dynorphin for 10 min prior to treatment with the indicated concentrations of orexin. Error bars represent standard error of the mean of three replicates and data shown is representative of 3 separate experiments. The mean EC₅₀s from the 3 separate experiments are quoted in the text.