Extended Data Figure 7.

Assessing OCR under basal and FCCP stimulated maximal respiration, and following NE stimulation + oligomycin in UCP1^−/− primary brown adipocytes transduced with UCP1 cysteine null mutants. a–g, Raw basal, maximal, and UCP1-dependent OCR from representative experiments of WT and UCP1^−/− brown adipocytes transduced with (a) UCP1 C24A (WT n = 11; C24A n = 12), (b) C188A (n = 7), (c) UCP1 C213A (n = 19), (d) UCP1 C287A (WT n = 9; C287A n = 10), (e) UCP1 C304A (WT n = 7; C304A n = 10), (f) UCP1 C224A (WT n = 9; C224A n = 10), (g) UCP1 C224A/C253A (n = 8). Data are mean ± s.e.m. of at least seven replicates. **P < 0.01, (two-tailed Student’s t-test for pairwise comparisons)