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. Author manuscript; available in PMC: 2017 Aug 9.
Published in final edited form as: Nature. 2016 Mar 30;532(7597):112–116. doi: 10.1038/nature17399

Figure 2. BAT mitochondrial ROS during thermogenesis drives oxidation of cellular and mitochondrial thiols.

Figure 2

a, Distribution of % oxidation status of BAT protein thiols ± acute cold exposure. b, Pathway analysis of BAT proteins containing cysteine residues sensitive to substantial oxidation (>10% shift in oxidation status) upon cold exposure. (Top) Proteins clustered according to shared GO enrichment terms. (Bottom) Significantly enriched pathways. c, Immunodetection of protein sulfenic acid levels in BAT ± acute cold exposure (n = 4) d, Effect of i.p. NAC on core body temperature following acute cold exposure (n = 8; 500 mg/kg NAC n = 7). e, VO2 consumed 3 hrs pre and post i.p. CL ± NAC (control n = 12; NAC n = 9). VCL = vinculin. Data are mean ± s.e.m. of at least four mouse replicates. *P < 0.05, **P < 0.01, ***P<0.001 (two-tailed Student’s t-test for pairwise comparisons, one-way/two-way ANOVA for multiple comparisons involving one/two independent variables).