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. Author manuscript; available in PMC: 2017 Aug 9.
Published in final edited form as: Nature. 2016 Mar 30;532(7597):112–116. doi: 10.1038/nature17399

Figure 3. BAT mitochondrial ROS oxidatively modify a cysteine residue on UCP1 and support UCP1-dependent leak respiration.

Figure 3

a,b, OCR of brown adipocytes under ± NE stimulation + oligomycin to determine leak respiration ± (a) MitoQ (n = 10) or (b) NAC (n = 8; 0.1 mM NAC n = 7). c, d OCR of primary UCP1−/− adipocytes under basal ± NE stimulation + oligomycin ± (c) MitoQ (n = 10) or (d) NAC (n = 10). e,f, Cys-redox immunoblot of BAT UCP1 (e) following a timecourse of acute cold exposure, and (f) following acute cold exposure ± 500 mg/kg NAC. FCCP = Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Data are mean ± s.e.m. of at least seven cellular replicates. *P < 0.05, **P < 0.01, (two-tailed Student’s t-test for pairwise comparisons, one-way ANOVA for multiple comparisons).