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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 26;507:231–241. doi: 10.1016/j.virol.2017.04.014

Fig. 2. Effect of decreased DDX6 abundance on HCV translation.

Fig. 2

Control and DDX6-specific siRNA transfected Huh7 cells were infected with JFH-1 at a moi of 0.01. Three days p.i. cells were harvested, and cell lysates layered onto a 10–60% sucrose polysome gradient. Protein and RNA complexes were separated in the gradient by ultracentrifugation and fractionation. (A) Polysome profile of Huh7 cells transfected with either control or DDX6 siRNAs, and infected with JFH-1. (B) Northern blot of the distribution of HCV RNA, and DDX6 and β-actin mRNAs in the polysome gradient. (C) Percent of HCV RNA distributed across the gradient. Data are from three independent experiments. Error bars display the standard error of the mean.