Fig. 3.

Examination of translation and replication of the subgenomic Renilla luciferase reporter JFH-1 replicon following depletion of DDX6. Replication competent (WT) and incompetent (GND) subgenomic reporter replicons were transfected and expressed in Huh7 cells transfected with either control or DDX6-specific siRNAs. Cells were harvested at 4, 8, 24 and 48 h post-transfection of the replicon genomes. Data are a representative of three experiments showing units of Renilla luciferase activity (presented on a log₁₀ scale) following transfection of the subgenomic replicons. Data from each independent experiment was performed in duplicate and are presented as standard deviation (SD) of the mean. n.s. not significant, *p < 0.05 and ***p < 0.0001 by unpaired Student’s t-test.