Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 26;507:231–241. doi: 10.1016/j.virol.2017.04.014

Fig. 5. Effect of decreased DDX6 abundance on the stability of the HCV RNA.

Fig. 5

JFH-1 infected Huh7 cells transfected with either control or DDX6-specific siRNAs were incubated with DMSO or 25 μM MK0608. Cells were harvested at the time of addition of the inhibitor, and 2, 4, 6, 8 and 24 h post-MK0806 treatment. (A) Northern blot shows HCV RNA and actin mRNA abundance over time. (B) Quantification of HCV RNA decay over time. The data presented are from four independent experiments. (C) Quantification of a second set of experiments showing HCV RNA decay over the same time period. The data presented are from two independent experiments. (B–C) HCV RNA was normalized to 1 at the 2 h time point. The graphical representation of the data was generated using a nonlinear regression (curve fit), and one phase decay analyses using the GraphPad Prism6 software. GraphPad software was also used to calculate of RNA half-life from the data presented in (B) and (C). The error bars display the mean ± SD.