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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 26;507:231–241. doi: 10.1016/j.virol.2017.04.014

Fig. 6. Effect on HCV gene expression following depletion of DDX6 and miR-122, or addition of exogenous miR-122.

Fig. 6

(A) Huh7 cells were transfected with control or DDX6-specific siRNAs alone, or in combination with antisense LNA oligonucleotides targeting miR-122 or miR-106b. Transfected Huh7 cells were infected with JFH-1 at a moi of 0.01. HCV RNA and protein abundance was examined 3 days post-infection by western and northern blot analysis. (B) Quantitation of the relative levels of HCV RNA. HCV RNA abundance was normalized to cells transfected in the absence of siRNA and LNA antisense oligonucleotides. Data are representative of two independent experiments. (C) Huh7 transfected with control or DDX6-specific siRNAs alone, or in combination with 10 nM, 50 nM or 100 nM miR-122 (and 100 nM miR-106b), were infected with JFH-1, and HCV gene expression examined by western and northern blot. (D) Quantitation of the relative levels of HCV RNA. HCV RNA abundance was normalized to JFH-1-infected cells transfected with no siRNA and 100 nM miR-106b. Data are representative of two independent experiments. Error bars display the mean ± SD.